Pseudomonas Aeruginosa clinical
Clinical Isolates of Pseudomona aeruginosa
Chronic bacterial infections pose significant public health problems and contribute to the rise and spread of multi-drug resistant strains, leading to an even higher burden on the health care system. The majority of antibiotics currently used were developed decades ago, and although they have been indispensable for the treatment of many infectious diseases, antibiotic resistance has arisen to nearly all of them. In particular, Pseudomonas aeruginosa is an important clinical pathogen that can colonize or infect multiple tissues or organs, and for which there is significant antibiotic resistance.
Our understanding of the pathogenesis of P. aeruginosa comes from studies of only a small number of laboratory adapted strains, and although this approach has provided a great deal of knowledge, it has not provided significant insight into better ways to effectively treat P. aeruginosa infections. Advances in high throughput genomic sequencing along with analysis of infection models present us with the opportunity to correlate specific genotypes to phenotypic differences and thus, to better understand and properly treat infections.
Comparative genomics of different strains of the same species can provide insights into the loss or gain of genes through horizontal gene transfer as well as the evolution of genes through changes at the nucleotide level. By analyzing the genomic content of subsets of strains, we may better understand their ability to cause disease. Strains can be subgrouped based on whether they cause chronic versus acute disease (which likely has a genetic basis), or based on their anatomic location in the host (which may or may not have a genetic association). Comparing the genomes of strains that cause acute versus chronic infection may allow us to uncover mechanisms used by the bacteria to cause the specific type of infection. For example, strains that cause chronic infections may encode certain proteins or upregulate certain transcriptional programs that allow them to evade host immune responses and persist within the organism. Furthermore, comparison of strains isolated from various anatomic locations (i.e. urinary tract, ocular or blood isolates) may reveal genes that are responsible for niche tropism or adaptation. Finally, genomic analysis of clinical isolates, whether acute or chronic, may provide information on novel mechanisms of antibiotic resistance.
Project Data can also be found at NCBI
Please cite all data relating to this initiative (including individual genes and genomes) as:
"Pseudomonas aeruginosa initiative, Broad Institute (broadinstitute.org)"